个人简介

• B.A. Dartmouth College (1998) • Ph.D. California Institute of Technology (2005) • NIH/NINDS Predoctoral Fellow (2004-2005) • NIH/NIGMS Postdoctoral Fellow, Yale University (2006-2008) • Searle Scholar (2010) • Sloan Research Fellow (2012) • NSF CAREER Award (2012)

研究领域

Biological/Chemical Biology/Organic

Protein motion underlies both proper function and disease in biological systems. Many signaling and transport proteins require complex rearrangements for function, and some proteins, such as amyloids, misfold into toxic conformations. Studying these protein motions not only aids our understanding of diverse biological phenomena, it also contributes to an important fundamental problem in biochemistry: understanding how motions propagate from one end of a protein to another. The Petersson laboratory is developing tools to address questions of how dynamic proteins mediate communication and how the cellular environment catalyzes protein misfolding, from detailed in vitro folding studies to modeling protein motion in living cells. These tools include novel chromophores, which we synthesize and incorporate into proteins through unnatural amino acid mutagenesis and synthetic protein ligation.

近期论文

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Inteins as Traceless Purification Tags for Unnatural Amino Acid Proteins Solongo Batjargal, Christopher R. Walters, and E. James Petersson J. Am. Chem. Soc. ASAP DOI:10.1021/ja5103019 Specific Modulation of Protein Activity by Using a Bioorthogonal Reaction John B. Warner, Anand K. Muthusamy, and E. James Petersson ChemBioChem 15 2508-2514 (2014) DOI:10.1002/cbic.201402423 Surface Effects Mediate Self-Assembly of Amyloid-β Peptides Yi-Chih Lin, E. James Petersson, and Zahra Fakhraai ACS Nano 8 10178–10186 (2014) DOI:10.1021/nn5031669 Characterization of the Lipid Binding Properties of Otoferlin Reveal Specific Interactions Between PI(4,5)P2 and the C2C and C2F Domains Murugesh Padmanarayana, Nicole Hams, Lee C. Speight, E. James Petersson, Ryan A. Mehl, and Colin P. Johnson Biochemistry 53 5023-5033 (2014) DOI:10.1021/bi5004469 Two-for-one designer labels E. James Petersson and John B. Warner Nat. Chem. 6 379-381 (2014) DOI:10.1038/nchem.1935 On the use of thioamides as fluorescence quenching probes for tracking protein folding and stability E. James Petersson, Jacob M. Goldberg, and Rebecca F. Wissner Phys. Chem. Chem. Phys. 16 6827-6837 (2014) DOI:10.1039/C3CP55525A Thioamide-Based Fluorescent Protease Sensors Jacob M. Goldberg, Xing Chen, Nataline Meinhardt, Doron C. Greenbaum, and E. James Petersson J. Am. Chem. Soc. 136 2086–2093 (2014) DOI:10.1021/ja412297x Minimalist Approaches to Protein Labelling: Getting the Most Fluorescent Bang for Your Steric Buck Lee C. Speight,* Moumita Samanta,* and E. James Petersson Aust. J. Chem. 67 686-700 (2014) DOI:10.1071/CH13554 Included in Aust. J. Chem. 2013 Physical Organic Chemistry Gordon Research Conference Issue. Synthesis of thioester peptides for the incorporation of thioamides into proteins by native chemical ligation Solongo Batjargal, Yun Huang, Yanxin J. Wang, and E. James Petersson J. Peptide Sci. 20 87-91 (2014) DOI:10.1002/psc.2589 Included in J. Peptide Sci. 2013 Chemical Protein Synthesis Meeting Issue. Efficient Synthesis and In Vivo Incorporation of Acridon-2-ylalanine, a Fluorescent Amino Acid for Lifetime and Förster Resonance Energy Transfer/Luminescence Resonance Energy Transfer Studies Lee C. Speight, Anand K. Muthusamy, Jacob M. Goldberg, John B. Warner, Rebecca F. Wissner, Taylor S. Willi, Bradley F. Woodman, Ryan A. Mehl, and E. James Petersson J. Am. Chem. Soc. 135 18806-18814 (2013) DOI:10.1021/ja403247j Thioamide Quenching of Fluorescent Probes through Photoinduced Electron Transfer: Mechanistic Studies and Applications Jacob M. Goldberg, Solongo Batjargal, Benson S. Chen, and E. James Petersson J. Am. Chem. Soc. 135 18651-18658 (2013) DOI:10.1021/ja409709x Efficient, Traceless Semi-Synthesis of α-Synuclein Labeled with a Fluorophore/Thioamide FRET Pair Rebecca F. Wissner, Anne M. Wagner, John B. Warner, and E. James Petersson Synlett 24 2454-2458 (2013) DOI:10.1055/s-0033-1339853 Included in Synlett 2013 EuCheMS Young Investigator Workshop Issue. Molecular basis for N-terminal acetylation by the heterodimeric NatA complex Glen Liszczak, Jacob M. Goldberg, Håvard Foyn, E. James Petersson, Thomas Arnesen, and Ronen Marmorstein Nat. Struct. Mol. Biol. 20 1098-1105 (2013) DOI:10.1038/nsmb.2636 Labeling Proteins with Fluorophore/Thioamide Förster Resonant Energy Transfer Pairs by Combining Unnatural Amino Acid Mutagenesis and Native Chemical Ligation Rebecca F. Wissner, Solongo Batjargal, Colin M. Fadzen, and E. James Petersson J. Am. Chem. Soc. 135 6529-6540 (2013) DOI:10.1021/ja4005943 Expressed Protein Ligation at Methionine: N-Terminal Attachment of Homocysteine, Ligation, and Masking Tomohiro Tanaka, Anne M. Wagner, John B. Warner, Yanxin J. Wang, and E. James Petersson Angew. Chem. Int. Ed. 52 6210-6213 (2013) DOI:10.1002/anie.201302065 Native Chemical Ligation of Thioamide-Containing Peptides: Development and Application to the Synthesis of Labeled α-Synuclein for Misfolding Studies Solongo Batjargal, Yanxin J. Wang, Jacob M. Goldberg, Rebecca F. Wissner, and E. James Petersson J. Am. Chem. Soc. 134 9172-9182 (2012) DOI:10.1021/ja2113245 Highlighted in C&E News: Chem. Eng. News 90 34 (2012). Spotlighted in JACS: J. Am. Chem. Soc. 134 6499 (2012). Minimalist Probes for Studying Protein Dynamics: Thioamide Quenching of Selectively Excitable Fluorescent Amino Acids Jacob M. Goldberg, Lee C. Speight, Mark W. Fegley, and E. James Petersson J. Am. Chem. Soc. 134 6088-6091 (2012) DOI: 10.1021/ja3005094 Highlighted in C&E News: Chem. Eng. News 90 34 (2012). Thioamide Quenching of Intrinsic Protein Fluorescence Jacob M. Goldberg, Rebecca F. Wissner, Alyssa M. Klein, and E. James Petersson Chem. Commun. 48 1550-1552 (2012) DOI:10.1039/C1CC14708K Included in Chem. Commun. 2012 Emerging Investigator Issue. N-Terminal Protein Modification Using Simple Aminoacyl Transferase Substrates Anne M. Wagner, Mark W. Fegley, John B. Warner, Christina L. J. Grindley, Nicholas P. Marotta, and E. James Petersson J. Am. Chem. Soc. 133 15139-15147 (2011) DOI:10.1021/ja2055098 Thioamides as Fluorescence Quenching Probes: Minimalist Chromophores to Monitor Protein Dynamics Jacob M. Goldberg, Solongo Batjargal, and E. James Petersson J. Am. Chem. Soc. 132 14718-14720 (2010) DOI:10.1021/ja1044924 For Professor Petersson's publications from Yale University or Caltech, click here to see his CV.