Abstract

The study demonstrates that the interaction of dopamine with fluorescamine can serve as a basis for a fluorescence assay for dopamine using a camera, as the product formed in this interaction exhibits a fluorescence peak in the visible spectral region (485 nm). Fluorescence can be excited with a light-emitting diode emitting in the near-ultraviolet spectral region (395 nm). The reaction should be carried out at pH 8–8.5 in a phosphate buffer solution for 5 min, with fluorescamine being added to the reaction mixture last. The performance characteristics of the camera-based assay were evaluated and compared with those of a similar dopamine assay using a professional spectrofluorometer and a spectrophotometer. The limits of detection for dopamine using a camera, a spectrophotometer, and a spectrofluorometer were 1.8, 1.6, and 0.5 µM, respectively, while the analytical ranges were 5.4–50, 4.8–100, and 1.5–100 µM. The presence of common inorganic ions in concentrations 10 times higher than that of dopamine does not interfere with the assay. The proposed method for dopamine determination can be used for the quality control of pharmaceutical products.

中文翻译：

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使用光电相机测定多巴胺的发光

 抽象

该研究表明，多巴胺与荧光胺的相互作用可以作为使用相机进行多巴胺荧光测定的基础，因为在这种相互作用中形成的产物在可见光谱区域 （485 nm） 中表现出荧光峰。荧光可以用在近紫外光谱区域 （395 nm） 发射的发光二极管激发。反应应在 pH 值为 8–8.5 的磷酸盐缓冲溶液中进行 5 分钟，最后将荧光虫胺加入反应混合物中。评估了基于相机的测定的性能特征，并使用专业荧光分光光度计和分光光度计与类似的多巴胺测定的性能特征进行了比较。使用相机、分光光度计和荧光分光光度计对多巴胺的检测限分别为 1.8、1.6 和 0.5 μM，而分析范围为 5.4-50、4.8-100 和 1.5-100 μM。浓度比多巴胺高 10 倍的常见无机离子的存在不会干扰测定。所提出的多巴胺测定方法可用于药品的质量控制。