Abstract

The principal aim of this investigation is to improve the precision and accuracy of quantifying sugars, such as glucose, sucrose, and fructose, through the utilization of hydrophilic interaction liquid chromatography (HILIC) with charged aerosol detection (CAD) in Pueraria tuberosa, a plant renowned for its pharmacological properties. Refractive index detectors are commonly employed to determine sugars in plant extracts. However, it is important to note that these detectors suffer from poor sensitivity and are prone to interference from co-eluting components. Therefore, alternative methods need to be explored to overcome these limitations. HPLC-CAD offers a promising solution by providing improved sensitivity, enhanced precision, and reduced interference from co-eluting sample components. Optimal separation of substances was achieved by implementing an Inertsil HILIC column (250 × 4.6 mm, 5 μm). The mobile phase consisted of 0.1% trifluoroacetic acid in acetonitrile and 0.1% trifluoroacetic acid in water. The flow rate was set at 0.8 mL/min. By employing a systematic approach to modify the chromatographic conditions, this study has successfully improved the sensitivity, resolution, and reliability of sugar determination.

中文翻译：

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通过应用经过分析验证的亲水作用液相色谱和电雾式检测法测定块茎葛根中的营养糖类

 抽象

本研究的主要目的是通过使用亲水作用液相色谱 （HILIC） 和电雾式检测 （CAD） 在块茎葛根中定量葡萄糖、蔗糖和果糖等糖类的精度和准确性，葛根是一种以其药理特性而闻名的植物。示差折光检测器通常用于测定植物提取物中的糖类。但是，需要注意的是，这些检测器的灵敏度较差，并且容易受到共洗脱组分的干扰。因此，需要探索替代方法来克服这些限制。HPLC-CAD 通过提高灵敏度、增强精密度并减少共洗脱样品组分的干扰，提供了一种有前途的解决方案。通过使用 Inertsil HILIC 色谱柱（250 × 4.6 mm、5 μm）实现物质的最佳分离。流动相由 0.1% 三氟乙酸的乙腈溶液和 0.1% 三氟乙酸的水溶液组成。流速设置为 0.8 mL/min。通过采用系统的方法改变色谱条件，本研究成功提高了糖度测定的灵敏度、分离度和可靠性。